mouse anti human tlr2 cd282 Search Results


tlr2 pe  (Miltenyi Biotec)
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Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction
Tlr2 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reafinity  (Miltenyi Biotec)
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Miltenyi Biotec reafinity
Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction
Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd282 tlr2 apc  (Miltenyi Biotec)
95
Miltenyi Biotec cd282 tlr2 apc
Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction
Cd282 Tlr2 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tlr2 pe vio615  (Miltenyi Biotec)
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Miltenyi Biotec tlr2 pe vio615
Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction
Tlr2 Pe Vio615, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tlr2 pe vio770  (Miltenyi Biotec)
95
Miltenyi Biotec tlr2 pe vio770
Co-stimulation with TLR1/2, <t>TLR2/6,</t> and TLR5 agonists enhances the proliferative response of human blood-derived CD8 + T cells to TCR triggering. (A) Expression profiles of surface TLRs in resting CD8 + T cells within human blood mononuclear cells (PBMCs). The T cell subpopulations of human PBMCs were identified by staining the cells with antibodies against CD3, CD4, and CD8; the TLR expression was measured by flow cytometry and expressed as the percentage of positively stained cells. Shown is aggregated data of 12 samples. (B and C) Co-stimulatory effects of different TLR agonists on the proliferation of human PBMC-derived CD8 + T cells. CD8+T cells were purified from human PBMCs, stained with eFluor 670, and then stimulated with ant-CD3 beads, anti-CD3/CD28 beads, or anti-CD3 beads coupled with the indicated TLRs agonists. Cells were harvested 7 days later and dilution of eFluor 670 was determined by flow cytometry to measure cellular proliferation, with representative flow cytometry plot and data summary (n = 3) shown in (B) and (C) , respectively. (D,E) Determination of optimal dosages of TLR agonists required for co-stimulation of ex vivo proliferation of human CD8 + T cells. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in presence of various concentration of TLR1/2 agonist, <t>TLR2/6</t> agonist, or TLR5 agonist after labelling with eFluor 670. Flow cytometry analyses were performed on day 4 and 6 after stimulation to determine the cellular proliferation through assessment of eFluor 670 dilution. Shown is aggregated data of 3 samples.
Tlr2 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated tlr2 antibody  (Miltenyi Biotec)
95
Miltenyi Biotec biotinylated tlr2 antibody
Co-stimulation with TLR1/2, <t>TLR2/6,</t> and TLR5 agonists enhances the proliferative response of human blood-derived CD8 + T cells to TCR triggering. (A) Expression profiles of surface TLRs in resting CD8 + T cells within human blood mononuclear cells (PBMCs). The T cell subpopulations of human PBMCs were identified by staining the cells with antibodies against CD3, CD4, and CD8; the TLR expression was measured by flow cytometry and expressed as the percentage of positively stained cells. Shown is aggregated data of 12 samples. (B and C) Co-stimulatory effects of different TLR agonists on the proliferation of human PBMC-derived CD8 + T cells. CD8+T cells were purified from human PBMCs, stained with eFluor 670, and then stimulated with ant-CD3 beads, anti-CD3/CD28 beads, or anti-CD3 beads coupled with the indicated TLRs agonists. Cells were harvested 7 days later and dilution of eFluor 670 was determined by flow cytometry to measure cellular proliferation, with representative flow cytometry plot and data summary (n = 3) shown in (B) and (C) , respectively. (D,E) Determination of optimal dosages of TLR agonists required for co-stimulation of ex vivo proliferation of human CD8 + T cells. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in presence of various concentration of TLR1/2 agonist, <t>TLR2/6</t> agonist, or TLR5 agonist after labelling with eFluor 670. Flow cytometry analyses were performed on day 4 and 6 after stimulation to determine the cellular proliferation through assessment of eFluor 670 dilution. Shown is aggregated data of 3 samples.
Biotinylated Tlr2 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated tlr2 antibody/product/Miltenyi Biotec
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Image Search Results


Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction

Journal: Immunology

Article Title: Differential regulation of Toll-like receptor signalling in spleen and Peyer's patch dendritic cells

doi: 10.1111/j.1365-2567.2010.03317.x

Figure Lengend Snippet: Gene-specific primers and probe combinations assayed by quantitative real-time polymerase chain reaction

Article Snippet: Flow cytometry Using a FACSCalibur (BD Biosciences, San Jose, CA) flow cytometer, the surface phenotype of cells was determined using four-colour staining with the following antibodies: fluorescein isothiocyanate-conjugated (-FITC) or allophycocyanin-conjugated CD11c (HL3), phycoerythrin-conjugated (-PE) CD80 (16-10A1) (BD-Pharmingen, Oxford, UK); TLR2-PE (6C2), TLR4/MD2-PE-Cy7 (MTS510) (eBioscience, San Diego, CA) and major histocompatibility class II (MHCII)-FITC (M5/114) (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Sequencing

A greater percentage of spleen dendritic cells (DCs) express surface Toll-like receptor 2 (TLR2) and TLR4 than Peyer's patch (PP) DCs. (a) AutoMACS-separated CD11c+ cells from spleen and PP were gated on the expression of CD11c and MHCII as in Fig. 1(a). The expression of surface TLR2 and TLR4-MD2 was then determined on the double-positive population. Values in the upper right of the histograms indicate the mean fluorescence intensity of cells in the indicated gate, n = 3. Bars represent mean ± SEM of percentage of cells falling in the indicated gate of the CD11c+ MHCII+ population, n = 3. (b) autoMACS-separated, flow cytometrically sorted DCs were analysed for gene expression of tlr 1–9. Expression was determined as fold induction compared with β-actin housekeeper. There was no statistical difference between spleen and PP in the messenger RNA levels of any of the genes investigated. Bars represent mean ± SEM, n = 4 or n = 5. Significance determined by unpaired Student's t-test, ***P < 0·001, *P < 0·05.

Journal: Immunology

Article Title: Differential regulation of Toll-like receptor signalling in spleen and Peyer's patch dendritic cells

doi: 10.1111/j.1365-2567.2010.03317.x

Figure Lengend Snippet: A greater percentage of spleen dendritic cells (DCs) express surface Toll-like receptor 2 (TLR2) and TLR4 than Peyer's patch (PP) DCs. (a) AutoMACS-separated CD11c+ cells from spleen and PP were gated on the expression of CD11c and MHCII as in Fig. 1(a). The expression of surface TLR2 and TLR4-MD2 was then determined on the double-positive population. Values in the upper right of the histograms indicate the mean fluorescence intensity of cells in the indicated gate, n = 3. Bars represent mean ± SEM of percentage of cells falling in the indicated gate of the CD11c+ MHCII+ population, n = 3. (b) autoMACS-separated, flow cytometrically sorted DCs were analysed for gene expression of tlr 1–9. Expression was determined as fold induction compared with β-actin housekeeper. There was no statistical difference between spleen and PP in the messenger RNA levels of any of the genes investigated. Bars represent mean ± SEM, n = 4 or n = 5. Significance determined by unpaired Student's t-test, ***P < 0·001, *P < 0·05.

Article Snippet: Flow cytometry Using a FACSCalibur (BD Biosciences, San Jose, CA) flow cytometer, the surface phenotype of cells was determined using four-colour staining with the following antibodies: fluorescein isothiocyanate-conjugated (-FITC) or allophycocyanin-conjugated CD11c (HL3), phycoerythrin-conjugated (-PE) CD80 (16-10A1) (BD-Pharmingen, Oxford, UK); TLR2-PE (6C2), TLR4/MD2-PE-Cy7 (MTS510) (eBioscience, San Diego, CA) and major histocompatibility class II (MHCII)-FITC (M5/114) (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Fluorescence, Gene Expression

Co-stimulation with TLR1/2, TLR2/6, and TLR5 agonists enhances the proliferative response of human blood-derived CD8 + T cells to TCR triggering. (A) Expression profiles of surface TLRs in resting CD8 + T cells within human blood mononuclear cells (PBMCs). The T cell subpopulations of human PBMCs were identified by staining the cells with antibodies against CD3, CD4, and CD8; the TLR expression was measured by flow cytometry and expressed as the percentage of positively stained cells. Shown is aggregated data of 12 samples. (B and C) Co-stimulatory effects of different TLR agonists on the proliferation of human PBMC-derived CD8 + T cells. CD8+T cells were purified from human PBMCs, stained with eFluor 670, and then stimulated with ant-CD3 beads, anti-CD3/CD28 beads, or anti-CD3 beads coupled with the indicated TLRs agonists. Cells were harvested 7 days later and dilution of eFluor 670 was determined by flow cytometry to measure cellular proliferation, with representative flow cytometry plot and data summary (n = 3) shown in (B) and (C) , respectively. (D,E) Determination of optimal dosages of TLR agonists required for co-stimulation of ex vivo proliferation of human CD8 + T cells. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in presence of various concentration of TLR1/2 agonist, TLR2/6 agonist, or TLR5 agonist after labelling with eFluor 670. Flow cytometry analyses were performed on day 4 and 6 after stimulation to determine the cellular proliferation through assessment of eFluor 670 dilution. Shown is aggregated data of 3 samples.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Improving the ex vivo expansion of human tumor-reactive CD8 + T cells by targeting toll-like receptors

doi: 10.3389/fbioe.2022.1027619

Figure Lengend Snippet: Co-stimulation with TLR1/2, TLR2/6, and TLR5 agonists enhances the proliferative response of human blood-derived CD8 + T cells to TCR triggering. (A) Expression profiles of surface TLRs in resting CD8 + T cells within human blood mononuclear cells (PBMCs). The T cell subpopulations of human PBMCs were identified by staining the cells with antibodies against CD3, CD4, and CD8; the TLR expression was measured by flow cytometry and expressed as the percentage of positively stained cells. Shown is aggregated data of 12 samples. (B and C) Co-stimulatory effects of different TLR agonists on the proliferation of human PBMC-derived CD8 + T cells. CD8+T cells were purified from human PBMCs, stained with eFluor 670, and then stimulated with ant-CD3 beads, anti-CD3/CD28 beads, or anti-CD3 beads coupled with the indicated TLRs agonists. Cells were harvested 7 days later and dilution of eFluor 670 was determined by flow cytometry to measure cellular proliferation, with representative flow cytometry plot and data summary (n = 3) shown in (B) and (C) , respectively. (D,E) Determination of optimal dosages of TLR agonists required for co-stimulation of ex vivo proliferation of human CD8 + T cells. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in presence of various concentration of TLR1/2 agonist, TLR2/6 agonist, or TLR5 agonist after labelling with eFluor 670. Flow cytometry analyses were performed on day 4 and 6 after stimulation to determine the cellular proliferation through assessment of eFluor 670 dilution. Shown is aggregated data of 3 samples.

Article Snippet: For surface staining, cells were stained in FACS buffer (PBS supplemented with 2% bovine serum albumin) sequentially with a fixable viability dye and a combination of the following antibodies: CD3-ECD (IM2705U, Beckman coulter), CD8-BV711(563677, BD Pharmingen), CD4-percp-cy5.5 (300530, Biolegend), CD14-BV786(563699, BD Pharmingen), TLR1-FITC(sc-47709FITC, Santa Cruz), TLR2-PE-Vio770 (130-099-021, Miltenyi), TLR4-BV421 (312811, Biolegend), TLR5-Alexa Fluor 700 (NBP2-24787AF700, NOVUS biology), TLR6-DyLight 350 (NB100-56536UV, NOVUS biology), CD3-BV421(562426, BD Pharmingen), CD8-APC-H7(580179, BD Pharmingen), PD-1-PE-CF594(565024, BD Pharmingen), CTLA-4-APC(555855, BD Pharmingen), ICOS-FITC(313505, Biolegend), 4-1BB-PE-Cy7 (309818, Biolegend).

Techniques: Derivative Assay, Expressing, Staining, Flow Cytometry, Purification, Ex Vivo, Concentration Assay

Co-stimulation with a combination of TLR1/2, TLR2/6, and TLR5 agonists enhances the transcriptional induction of proliferative-related genes in human blood-derived CD8 + T cells responding to TCR triggering. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in the presence or absence of a combination of TLR1/2 agonist, TLR2/6 agonist, and TLR5 agonist. Cells were harvested 24 h later for extracting total RNA, which was subjected to RNA sequencing. The differentially expressed genes showing >2-fold change between minus and plus TLR agonists groups were clustered based on Gene Ontology (GO) enrichment analysis (A) . Shown in (B) is the heatmap of representative proliferation-related genes that were identified to be co-stimulated by TLR agonists. H1 to H3 represent samples from three healthy individuals.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Improving the ex vivo expansion of human tumor-reactive CD8 + T cells by targeting toll-like receptors

doi: 10.3389/fbioe.2022.1027619

Figure Lengend Snippet: Co-stimulation with a combination of TLR1/2, TLR2/6, and TLR5 agonists enhances the transcriptional induction of proliferative-related genes in human blood-derived CD8 + T cells responding to TCR triggering. CD8+T cells purified from human PBMCs were stimulated with anti-CD3 beads in the presence or absence of a combination of TLR1/2 agonist, TLR2/6 agonist, and TLR5 agonist. Cells were harvested 24 h later for extracting total RNA, which was subjected to RNA sequencing. The differentially expressed genes showing >2-fold change between minus and plus TLR agonists groups were clustered based on Gene Ontology (GO) enrichment analysis (A) . Shown in (B) is the heatmap of representative proliferation-related genes that were identified to be co-stimulated by TLR agonists. H1 to H3 represent samples from three healthy individuals.

Article Snippet: For surface staining, cells were stained in FACS buffer (PBS supplemented with 2% bovine serum albumin) sequentially with a fixable viability dye and a combination of the following antibodies: CD3-ECD (IM2705U, Beckman coulter), CD8-BV711(563677, BD Pharmingen), CD4-percp-cy5.5 (300530, Biolegend), CD14-BV786(563699, BD Pharmingen), TLR1-FITC(sc-47709FITC, Santa Cruz), TLR2-PE-Vio770 (130-099-021, Miltenyi), TLR4-BV421 (312811, Biolegend), TLR5-Alexa Fluor 700 (NBP2-24787AF700, NOVUS biology), TLR6-DyLight 350 (NB100-56536UV, NOVUS biology), CD3-BV421(562426, BD Pharmingen), CD8-APC-H7(580179, BD Pharmingen), PD-1-PE-CF594(565024, BD Pharmingen), CTLA-4-APC(555855, BD Pharmingen), ICOS-FITC(313505, Biolegend), 4-1BB-PE-Cy7 (309818, Biolegend).

Techniques: Derivative Assay, Purification, RNA Sequencing

TLR agonists is an advantageous supplement benefiting the ex vivo expansion of human blood-derived PD1+ CD8 + T cells. (A) Exploration of optimal combination of TLR agonists in facilitating the ex vivo expansion of human blood-derived PD1+ CD8 + T cells. PD1+ CD8 + T cells isolated from blood samples of health individuals were continuously cultured for 21 days in medium containing anti-CD3/anti-CD28 beads and IL-7/IL-15, either alone or in the presence of different combination of TLR1/2, TLR2/6, and TLR5 agonists. Cell counting was performed at various time points throughout the culture period to generate the cell expansion curve. (B) Expansion curves of PD1+ CD8 + T cells derived from blood samples of cancer patients with different tumor types. Among the total of fifteen samples, seven were with lung cancer, four with pancreatic cancer, and the rest four with other types of cancers. Five samples from healthy individuals were also included as controls.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Improving the ex vivo expansion of human tumor-reactive CD8 + T cells by targeting toll-like receptors

doi: 10.3389/fbioe.2022.1027619

Figure Lengend Snippet: TLR agonists is an advantageous supplement benefiting the ex vivo expansion of human blood-derived PD1+ CD8 + T cells. (A) Exploration of optimal combination of TLR agonists in facilitating the ex vivo expansion of human blood-derived PD1+ CD8 + T cells. PD1+ CD8 + T cells isolated from blood samples of health individuals were continuously cultured for 21 days in medium containing anti-CD3/anti-CD28 beads and IL-7/IL-15, either alone or in the presence of different combination of TLR1/2, TLR2/6, and TLR5 agonists. Cell counting was performed at various time points throughout the culture period to generate the cell expansion curve. (B) Expansion curves of PD1+ CD8 + T cells derived from blood samples of cancer patients with different tumor types. Among the total of fifteen samples, seven were with lung cancer, four with pancreatic cancer, and the rest four with other types of cancers. Five samples from healthy individuals were also included as controls.

Article Snippet: For surface staining, cells were stained in FACS buffer (PBS supplemented with 2% bovine serum albumin) sequentially with a fixable viability dye and a combination of the following antibodies: CD3-ECD (IM2705U, Beckman coulter), CD8-BV711(563677, BD Pharmingen), CD4-percp-cy5.5 (300530, Biolegend), CD14-BV786(563699, BD Pharmingen), TLR1-FITC(sc-47709FITC, Santa Cruz), TLR2-PE-Vio770 (130-099-021, Miltenyi), TLR4-BV421 (312811, Biolegend), TLR5-Alexa Fluor 700 (NBP2-24787AF700, NOVUS biology), TLR6-DyLight 350 (NB100-56536UV, NOVUS biology), CD3-BV421(562426, BD Pharmingen), CD8-APC-H7(580179, BD Pharmingen), PD-1-PE-CF594(565024, BD Pharmingen), CTLA-4-APC(555855, BD Pharmingen), ICOS-FITC(313505, Biolegend), 4-1BB-PE-Cy7 (309818, Biolegend).

Techniques: Ex Vivo, Derivative Assay, Isolation, Cell Culture, Cell Counting